Research Article: Cloning and partial characterization of the Methanococcoides burtonii minichromosome maintenance (MCM) helicase

Jae-Ho Shin; Rachel Mauro; Eugene Melamud; Rajesh Kasiviswanathan

doi:10.1893/0005-3155(2006)77[37:RACAPC]2.0.CO;2

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Jae-Ho Shin,¹ Rachel Mauro,^1,2 Eugene Melamud,¹ Rajesh Kasiviswanathan¹

¹aUnder the supervision of Zvi Kelman, University of Maryland Biotechnology Institute, Center for Adv
²bThomas S. Wootton HIgh School, 2100 Wootton Parkway, Rockville, MD 20850

Abstract

Minichromosome maintenance (MCM) helicases play an essential role in chromosomal DNA replication in archaea and eukarya. Here the isolation and partial characterization of the MCM homologue from the psychophilic archaeaon Methanococcoides burtonii is described. The protein was expressed and purified to near homogeneity from Escherichia coli and, like MCM homologues from other archaeons, the protein was shown to form hexamers.

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Jae-Ho Shin, Rachel Mauro, Eugene Melamud, and Rajesh Kasiviswanathan "Research Article: Cloning and partial characterization of the Methanococcoides burtonii minichromosome maintenance (MCM) helicase," BIOS 77(2), 37-41, (1 May 2006). https://doi.org/10.1893/0005-3155(2006)77[37:RACAPC]2.0.CO;2

Received: 1 November 2004; Accepted: 1 March 2005; Published: 1 May 2006

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